What does denaturation of proteins mean?

• The 3D structure of the enzyme has changed.

• The enzyme has increased ability to attach to the substrate.

• The substrate has changed.

How long does denaturation affect the enzyme?

• permanently

• temporarily

Look at the graph...What is the optimal temperature for this enzyme?

• 60 degrees C

• 15 degrees C

• 45 degrees C

• 35 degrees C

Warm-blooded mammals have an optimum temperature of

• 37 degrees C

• less than 20 degrees C

• about 45 degrees C

• greater than 50 degrees C

What leads to the change in shape of the active site when temperature increases?

• Hydrogen bonds between the amino acids break

• different amino acids in the enzyme

• increase in temperature, means more molecular collisions at the active site

Which Enzyme has an optimal pH that is Acidic?

• Pepsin

• Salivary amylase

• Arginase

Which enzyme has an optimum pH that is neutral (ph 7)

• Pepsin

• Salivary amylase 

• Arginase

Which enzyme has an optimum pH that is alkaline/Basic (pH above 7)

• Pepsin

• Salivary amylase

• Arginase 

What leads to the changes in shape of the active site when pH changes?

• more H⁺ interfere with the amino acid bonds

• the OH- ions break apart the amino acids