Exploring Cell Study Techniques: Microscopes and Magnification

The video tutorial covers the basics of microscopes, focusing on types, magnification, and calibration. It explains the differences between optical and electron microscopes, including their magnification and resolution capabilities. The tutorial also provides a detailed guide on calculating magnification and calibrating the eyepiece graticule, essential skills for studying cells.

What is the definition of magnification in microscopy?

• The number of times larger the image is compared to the actual object.

• The wavelength of light used in the microscope.

• The ability to distinguish two close objects as separate.

• The clarity of the image produced.

Which type of microscope uses a beam of electrons to create an image?

• Electron microscope

• Compound microscope

• Light microscope

• Optical microscope

What is a key advantage of using an electron microscope over an optical microscope?

• Color imaging

• Lower cost

• Higher magnification and resolution

• Ability to view living samples

Why can't living samples be viewed with an electron microscope?

• They produce color images.

• They are too small.

• They require a vacuum environment.

• They need to be stained.

What is the formula for calculating magnification?

• Actual size = Image size / Magnification

• Image size = Actual size / Magnification

• Magnification = Image size / Actual size

• Image size = Magnification / Actual size

How do you convert millimeters to micrometers?

• Multiply by 1000

• Multiply by 100

• Divide by 1000

• Divide by 100

What is an eyepiece graticule?

• A type of electron microscope

• A scale on the stage of the microscope

• A glass disk with a scale etched onto it

• A method for staining samples

Why is it necessary to calibrate the eyepiece graticule?

• To focus the microscope

• To change the color of the image

• To ensure accurate measurements at different magnifications

• To adjust the light intensity

What is the purpose of a stage micrometer?

• To measure the wavelength of light

• To calibrate the eyepiece graticule

• To magnify the image

• To stain the sample

What is the resolving power of a microscope determined by?

• The wavelength of the light or electrons used

• The type of sample

• The color of the image

• The magnification

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