Exploring Microscope Techniques in STEM

Professor Murry Gans introduces students to the non-majors biology lab at Eastfield College, focusing on the use of a light microscope. The video covers how to handle and set up the microscope, identify its parts, and prepare a wet mount slide. It also explains focusing techniques and proper storage after use. The video emphasizes the importance of starting with low power magnification and gradually increasing to high power, ensuring the specimen is centered. Students are encouraged to come prepared to class by reading relevant materials beforehand.

What is the correct way to carry a microscope?

• By the objective lenses

• By the base only

• By the eyepiece

• By the arm and supporting the base

What is the magnification power of the eyepieces or oculars?

• 4x

• 100x

• 10x

• 40x

Which part of the microscope is used to control the amount of light reaching the specimen?

• Stage

• Diaphragm

• Fine focus knob

• Coarse focus knob

What is the purpose of using lens paper before using the microscope?

• To clean the table

• To clean the lenses

• To clean the slides

• To clean the specimen

What is the technique called when mounting specimens in water?

• Permanent mount

• Dry mount

• Wet mount

• Temporary mount

Why do we start with the low power objective when focusing the microscope?

• It has the largest field of view

• It is the most powerful

• It is the easiest to use

• It has the smallest field of view

What should you do if you see nothing when switching to high power?

• Change the slide

• Use the coarse focus knob

• Increase the light intensity

• Center the specimen

What should be done with the cover slip after using the microscope?

• Wash and reuse it

• Dispose of it

• Store it in the slide box

• Leave it on the microscope

What is the final step in storing the microscope after use?

• Return the slide

• Turn off the light

• Unplug the scope

• Wrap the cord and cover the scope

What should you do if you miss something in the video tutorial?

• Guess the information

• Skip the part

• Watch the video again

• Ask the instructor