Common Mistakes in Microscope Use

The video discusses the 10 most common mistakes beginners make in microscopy. It emphasizes starting with low power objectives, centering the slide, adjusting the condenser properly, and using oil immersion correctly. It also highlights the importance of not confusing immersion oil with mounting medium, avoiding coarse focus with high power, and maintaining the microscope by keeping it dust-free and handling halogen bulbs with care. The video aims to help students and beginners improve their microscopy skills.

Why is it not recommended to start microscopy with high power objectives?

• They are easier to focus.

• They are less powerful.

• They have a very low depth of field.

• They provide a large depth of field.

What should you always check before using the microscope?

• The label of the slide.

• The position of the specimen over the condenser lens.

• The type of objective being used.

• The amount of immersion oil.

What is the effect of starting microscopy with the condenser fully open?

• Increases contrast.

• Decreases depth of field.

• Improves focus.

• Reduces light.

Which objective should be used with immersion oil?

• 100x oil immersion objective

• 40x objective

• 10x objective

• 4x objective

What is the consequence of confusing immersion oil with mounting medium?

• It improves image quality.

• It can damage the objective.

• It makes the slide temporary.

• It increases the depth of field.

What can happen if you use the coarse focus knob with high power objectives?

• It improves focus.

• It can damage the objective and slide.

• It increases the depth of field.

• It reduces light intensity.

What should you do if you lose focus while using a high power objective?

• Open the condenser fully.

• Switch to a low power objective and refocus.

• Quickly spin the fine focus knob.

• Increase the light intensity.

How should you control light intensity in microscopy?

• By using the light intensity knob.

• By opening the condenser fully.

• By adjusting the coarse focus knob.

• By using immersion oil.

Why is it important to keep your microscope covered?

• To keep it clean from dust.

• To prevent it from getting scratched.

• To improve image quality.

• To make it look new.

What should you do before switching on a microscope with a halogen light bulb?

• Use immersion oil.

• Set the light intensity to high.

• Open the condenser fully.

• Turn down the light intensity.