Microtomy Techniques and Procedures

This video tutorial covers the process of microtomy, focusing on the rotary microtome. It begins with an introduction to microtomy and its types, followed by detailed steps on preparing a water bath for histological sections. The tutorial then explains how to identify and mark slides, set up the microtome, and perform cuts. It concludes with finalizing the slides for analysis and emphasizes the importance of cleaning and safety measures post-experiment.

What are the two basic types of microtomes?

• Corrective and Sliding

• Sliding and Vibrating

• Rotary and Corrective

• Rotary and Vibrating

Why is distilled water used in the preparation of histological sections?

• To stain the sections

• To position the sections on the water surface

• To cool the sections

• To clean the sections

What is the purpose of heating the distilled water?

• To sterilize the water

• To make the paraffin sections easier to handle

• To evaporate the water

• To dissolve the paraffin

How is a slide identified before use?

• By marking it with a pen

• By attaching a label

• By using a barcode

• By scratching it

What is the first step in setting up the microtome?

• Positioning the blade

• Selecting the slide

• Locking the handwheel

• Heating the paraffin block

What is the purpose of the coarse cutting lever?

• To make fine adjustments

• To remove excess paraffin

• To heat the paraffin block

• To position the slide

What is the typical thickness range for fine cuts?

• 1 to 3 microns

• 21 to 30 microns

• 11 to 20 microns

• 4 to 10 microns

What should be done with the sections after cutting?

• They should be placed in a drying oven

• They should be discarded

• They should be stored in a freezer

• They should be stained immediately

Why is it important to clean the workbench after finishing the experiment?

• To avoid accidents

• To make space for the next user

• All of the above

• To prevent contamination

What is the final step after completing the microtomy process?

• Cleaning the blade

• Removing the paraffin block

• All of the above

• Locking the handwheel