Microscope Slide Preparation Techniques

The video tutorial guides viewers through the process of preparing a wet mount slide using a small letter 'e' from a newspaper. It explains how to place the letter on a slide, add water, and cover it with a slip to avoid air bubbles. The tutorial then demonstrates how to observe the slide under a microscope, adjust the power settings, and use the fine adjustment knob to focus on the newspaper fibers. It also provides an overview of the microscope's components, including the diaphragm, eyepiece, and objective lenses, and their respective magnifications.

What is the first step in preparing a wet mount slide?

• Place the cover slip on the slide

• Find and cut out a small letter 'e' from a newspaper

• Add water to the slide

• Adjust the microscope to high power

How should you handle the letter 'e' after cutting it out?

• With a microscope

• With a magnifying glass

• With tweezers

• With your fingers

What should you do if there are air bubbles under the cover slip?

• Ignore the bubbles

• Inject more water under the slide

• Remove the cover slip and start over

• Add more newspaper pieces

What is the purpose of using a paper towel during slide preparation?

• To clean the slide

• To absorb excess water

• To dry the letter 'e'

• To polish the cover slip

When observing the slide, what should you note about the letter 'e'?

• Its texture

• Its size

• Its orientation

• Its color

How do you move the slide to the lower left on the microscope stage?

• Move the slide to the lower right

• Move the slide to the upper left

• Move the slide to the lower left

• Move the slide to the upper right

What should you use to focus on a spot at high power?

• The fine adjustment knob

• The diaphragm

• The eyepiece

• The coarse adjustment knob

What can you observe at high power on the microscope?

• The whole letter 'e'

• The individual fibers of the newspaper

• The entire slide

• The entire newspaper

What is the function of the diaphragm on a microscope?

• To magnify the image

• To adjust the focus

• To control the amount of light

• To hold the slide in place

What is the magnification of the high power objective lens?

• 10 times

• 4 times

• 100 times

• 40 times