Cell Counting Techniques and Microscopy

This video tutorial provides detailed instructions on setting up and using a microscope for examining stained slides. It covers focusing techniques using different objectives, including 4x, 10x, 40x, and 100x, and explains the process of using oil immersion. The tutorial also guides viewers on how to count and differentiate white blood cells, emphasizing the importance of proper slide movement and cell identification. Key cell types such as neutrophils, lymphocytes, and monocytes are identified and recorded using a cell counter.

What should be done to the condenser and iris when preparing to view stained slides?

• Raise the condenser and open the iris

• Lower the condenser and close the iris

• Raise the condenser and close the iris

• Lower the condenser and open the iris

At what magnification should you start focusing on a slide?

• 10x

• 40x

• 4x

• 100x

What is the highest magnification you can use without oil?

• 10x

• 100x

• 4x

• 40x

Why is it important to find an area where red blood cells are just touching and slightly overlapping?

• To ensure the slide is clean

• To avoid using too much oil

• To find the perfect area for differential

• To prevent the slide from breaking

What should you avoid when counting white blood cells?

• Counting too quickly

• Moving the slide too slowly

• Double counting cells

• Using the coarse adjustment knob

What is a characteristic feature of a monocyte?

• Red color

• Kidney bean-shaped nucleus

• Round nucleus

• Small cytoplasm

How should you record a segmented neutrophil?

• By using a cell counter

• By writing it down

• By memorizing it

• By taking a photo

What is the purpose of following a systematic pattern when counting cells?

• To avoid using oil

• To save time

• To ensure accurate counting

• To make it easier

What should you do if you reach the edge of the slide while counting?

• Increase the magnification

• Move the slide one field horizontally

• Start over

• Stop counting

What indicates that you have reached 100 white blood cells?

• The cell counter dings

• The slide changes color

• The microscope shuts off

• A light turns on