Microscope Function and Operation

This video tutorial provides a comprehensive guide on assembling and using a light microscope. It covers the removal and installation of ocular and objective lenses, explains the optical and mechanical components, and demonstrates the correct handling and focusing techniques. The tutorial also includes practical steps for observing objects under different magnifications, using mirrors for light adjustment, and calculating total magnification.

What is the first step in assembling a light microscope?

• Focusing the eyepiece

• Adjusting the diaphragm

• Removing the cover from the eyepiece holder

• Attaching the objective lens

Which part of the microscope is closest to the eye?

• Eyepiece

• Stage

• Diaphragm

• Objective lens

What is the function of the coarse focus knob?

• To rotate the objective lenses

• To raise or lower the microscope tube

• To adjust the light intensity

• To move the stage left and right

How do you adjust the light intensity on a microscope?

• By changing the eyepiece

• By rotating the diaphragm

• By moving the stage

• By adjusting the coarse focus knob

Which part of the microscope holds the slide in place?

• Eyepiece

• Objective lens

• Stage clips

• Diaphragm

What is the purpose of the diaphragm on a microscope?

• To hold the eyepiece

• To adjust the light intensity

• To rotate the objective lenses

• To support the microscope

What is the function of the fine focus knob?

• To adjust the light intensity

• To make small adjustments to focus

• To change the objective lens

• To move the stage up and down

What should you do if the image is too dark when using a microscope?

• Use the concave mirror

• Use the flat mirror

• Lower the stage

• Increase the magnification

Which mirror should be used when the external light is sufficient?

• No mirror

• Concave mirror

• Flat mirror

• Both mirrors

What is the total magnification if both the eyepiece and objective lens magnify 10 times?

• 20 times

• 10 times

• 200 times

• 100 times