Vacuoles and Staining Techniques

This video tutorial introduces the structure and functions of cells, focusing on vacuoles. It includes an activity to observe vacuoles in a cactus leaf. The process involves preparing a thin cross-section of a cactus stem, staining it with safranin, and observing it under a microscope. The tutorial explains that vacuoles are large, fluid-filled spaces in cells, more prominent in plant cells than in animal cells. In mature plant cells, vacuoles occupy most of the cell space.

What is the main focus of the activity introduced in the chapter?

• Observing chloroplasts in a leaf

• Examining the roots of a cactus

• Studying the structure of a cactus flower

• Observing vacuoles in a cactus leaf

Which solution is used to stain the cactus stem section?

• Dilute safranin solution

• Eosin solution

• Iodine solution

• Methylene blue solution

What is the purpose of using a coverslip in the preparation of the slide?

• To add color to the specimen

• To keep the specimen flat and in place

• To protect the microscope lens

• To magnify the specimen

What are vacuoles primarily filled with?

• Fluid

• Gas

• Solid particles

• Air

How do vacuoles in animal cells generally compare to those in plant cells?

• They are smaller in animal cells

• They are larger in animal cells

• They are the same size in both

• They are absent in animal cells