Aseptic Technique & Streak Plate

What side of the plate is she labeling?

• the lid

• the bottom, the agar side

What is the purpose of flaming the loop after applying bacteria to the first quadrant?

• to prevent it from being cross-contaminated with any other bacteria present in the environment

• to remove all bacteria from it because you are trying to dilute the bacteria as you move from one quadrant to the next

• both of these are correct

In the last quadrant, what are you hoping to find later?

• a thick line of bacterial growth

• single isolated colonies

Which way do we incubate the plate?

• right side up

• upside down

What is the potential problem you notice here with the way she is holding hte loop right now?

Why do you never return to collect more bacteria from the bottle (or tube)?

• we are trying to dilute the sample

• you do collect more each time