Microscopy Concepts and Techniques

The video introduces the concept of protists and the necessity of microscopes to observe them. It explains key terms like magnification and resolution, and describes different types of microscopes, including light and electron microscopes. The video provides a detailed guide on using a brightfield microscope, including its components and functions. It concludes with practical tips for handling microscopes and preparing specimens, encouraging exploration of the microscopic world.

Why did Pinky place the protists on the kitchen table?

• To observe them with a microscope

• To clean the table

• To use them for cooking

• To dry them out

What is the significance of resolution in microscopy?

• It determines the color of the image

• It helps distinguish between two close objects

• It changes the shape of the specimen

• It increases the size of the image

Which type of microscope is commonly found in classrooms?

• Brightfield light microscope

• Scanning electron microscope

• Phase-contrast microscope

• Electron microscope

What is the main difference between TEM and SEM?

• TEM is smaller than SEM

• TEM is for internal structures, SEM for 3D surfaces

• TEM is used for surface visualization, SEM for internal structures

• TEM uses light, SEM uses electrons

What is the purpose of the condenser lens in a brightfield microscope?

• To magnify the image

• To focus light on the specimen

• To change the color of the light

• To hold the slide in place

How do you calculate total magnification in a compound microscope?

• Multiply the objective lens magnification by the eyepiece lens magnification

• Subtract the eyepiece magnification from the objective lens magnification

• Divide the eyepiece magnification by the objective lens magnification

• Add the magnifications of all lenses

What is the function of the coarse focus knob?

• To make large adjustments in focus

• To change the magnification level

• To adjust the light intensity

• To move the stage side to side

What should you use to clean the microscope lens?

• Cotton cloth

• Lens paper

• Paper towel

• Regular tissue

What is a wet mount used for?

• To dry the specimen

• To prepare a slide with liquid samples

• To stain the specimen

• To magnify the specimen

What should you do before storing a microscope?

• Leave the slide on the stage

• Keep the light on

• Cover the microscope

• Raise the stage to its highest position