Microscopy Concepts and Techniques

To observe them with a microscope

To clean the table

To use them for cooking

To dry them out

It determines the color of the image

It helps distinguish between two close objects

It changes the shape of the specimen

It increases the size of the image

Brightfield light microscope

Scanning electron microscope

Phase-contrast microscope

Electron microscope

TEM is smaller than SEM

TEM is for internal structures, SEM for 3D surfaces

TEM is used for surface visualization, SEM for internal structures

TEM uses light, SEM uses electrons

To magnify the image

To focus light on the specimen

To change the color of the light

To hold the slide in place

Multiply the objective lens magnification by the eyepiece lens magnification

Subtract the eyepiece magnification from the objective lens magnification

Divide the eyepiece magnification by the objective lens magnification

Add the magnifications of all lenses

To make large adjustments in focus

To change the magnification level

To adjust the light intensity

To move the stage side to side

Cotton cloth

Lens paper

Paper towel

Regular tissue

To dry the specimen

To prepare a slide with liquid samples

To stain the specimen

To magnify the specimen

Leave the slide on the stage

Keep the light on

Cover the microscope

Raise the stage to its highest position