DNAse Test | Theory & Results

The video tutorial by Kevin Tolkoff explains the DNA test, focusing on DNA and DNAse enzymes. It covers the structure of DNA, the role of DNAse in breaking down DNA into nucleotides, and the formation of the DNA methyl green complex. The tutorial also differentiates between DNA positive and negative organisms, highlighting the visual indicators of DNA breakdown on agar plates.

What is the primary role of DNA in most organisms?

• To transport nutrients

• To store genetic information

• To provide energy

• To act as a structural component

Which component is NOT part of a DNA unit?

• Phosphate

• Deoxyribose

• Base

• Amino acid

What happens to a DNA strand when DNAse acts on it?

• It remains unchanged

• It becomes longer

• It breaks down into smaller units

• It changes color

What is the role of methyl green in the DNAse test?

• To provide nutrients

• To act as an indicator

• To form DNA strands

• To break down DNA

What indicates a DNAse-positive organism in the test?

• A green color around the streak

• A red color around the streak

• A halo or clearing around the streak

• No change in color

Why do free nucleotides not appear green in the DNAse test?

• They are too small to be seen

• They are washed away by water

• They absorb the green color

• They do not complex with methyl green

What is the purpose of washing the agar with hydrochloric acid in the DNAse test?

• To wash away free nucleotides

• To enhance the green color

• To dissolve the DNA

• To sterilize the agar