UV-Vis Spectroscopy: Theory and "How do I know when to use it?"

The video tutorial covers the basics of UV VIS spectroscopy, focusing on the absorption properties of various molecules. It explains the significance of double bonds and conjugation in determining a molecule's ability to absorb UV light. The tutorial also delves into electron transitions, particularly π to π star transitions, and their role in spectroscopy. Additionally, it outlines the UV VIS spectroscopy technique, including the use of a cuvet and light source, and discusses the relationship between energy and wavelength. The video concludes with potential applications in biochemistry, such as identifying proteins and nucleic acids.

What is the significance of conjugation in molecules for UV-Vis spectroscopy?

• It increases the molecule's weight.

• It enhances the molecule's color.

• It affects the molecule's absorption properties.

• It changes the molecule's shape.

Why is butane not suitable for UV-Vis spectroscopy?

• It is too volatile.

• It is too large.

• It lacks double bonds.

• It is too reactive.

Which type of electrons are primarily involved in absorbing light in UV-Vis spectroscopy?

• Pi electrons

• Valence electrons

• Core electrons

• Sigma electrons

What happens to an electron when it absorbs light in UV-Vis spectroscopy?

• It disappears.

• It becomes a proton.

• It moves to a lower energy state.

• It gets excited to a higher energy state.

How are energy and wavelength related in UV-Vis spectroscopy?

• They are directly proportional.

• They are unrelated.

• They are equal.

• They are inversely proportional.

What type of transition is most useful in UV-Vis spectroscopy?

• Sigma to Sigma*

• Pi to Pi*

• N to Pi*

• Core to Valence

Which molecules tend to absorb at higher wavelengths?

• Molecules with no double bonds

• Molecules with isolated double bonds

• Molecules with only single bonds

• Molecules with significant conjugation

What is a practical application of UV-Vis spectroscopy in biochemistry?

• Detecting proteins with specific coenzymes

• Identifying the color of solutions

• Measuring the weight of proteins

• Determining the pH of a solution

How can UV-Vis spectroscopy be used to study DNA?

• By identifying its specific wavelength of absorption

• By measuring its weight

• By analyzing its pH

• By determining its color

What is the role of Woodward-Fieser rules in UV-Vis spectroscopy?

• To calculate the weight of molecules

• To determine the color of solutions

• To calculate theoretical wavelengths of absorption

• To measure the pH of solutions