Performing Column Chromatography

The video tutorial explains chromatography, focusing on column chromatography. It covers the basics of chromatography, the components and preparation of a column, and the process of loading and running samples. The tutorial also discusses the collection and analysis of fractions, and touches on advanced techniques like FPLC. Key concepts include the role of stationary and mobile phases, the importance of solvent management, and the separation of compounds based on polarity.

What is the primary difference between TLC and column chromatography?

• TLC can handle larger samples than column chromatography.

• Column chromatography is only used in biology, not chemistry.

• TLC uses a 3D matrix, while column chromatography uses a 2D surface.

• Column chromatography can separate larger sample quantities than TLC.

What is the role of silica gel in column chromatography?

• It acts as the mobile phase.

• It serves as the stationary phase.

• It is the solvent used in the process.

• It is used to clean the column.

Why is it important to keep the column wet during chromatography?

• To make the compounds more visible.

• To speed up the separation process.

• To ensure the stationary phase does not crack.

• To prevent the column from overheating.

What is the purpose of adding a layer of sand on top of the cotton ball in the column?

• To create a level surface for the silica gel.

• To filter out impurities from the sample.

• To act as an additional stationary phase.

• To prevent the solvent from evaporating.

What is the advantage of using colored chemicals like methyl red and bromo cressal green in this experiment?

• They are less likely to mix with each other.

• They require less solvent for separation.

• They are easier to visualize as they move through the column.

• They react faster with the stationary phase.

What is the main reason for collecting fractions in separate containers during column chromatography?

• To speed up the separation process.

• To ensure the compounds do not mix again.

• To reduce the amount of solvent used.

• To prevent the solvent from evaporating.

How can you determine the contents of each fraction if the compounds are not visible?

• By using a colorimetric assay.

• By using a microscope.

• By performing TLC on each fraction.

• By measuring the weight of each fraction.

What is a potential benefit of using multiple solvent systems in column chromatography?

• It decreases the cost of the experiment.

• It allows for the separation of more complex mixtures.

• It makes the process more environmentally friendly.

• It reduces the time needed for separation.

What is FPLC primarily used for in chromatography?

• Separating inorganic compounds.

• Separating proteins.

• Separating small organic molecules.

• Separating DNA strands.

Which of the following materials can be used in the column for specific separations?

• Only organic solvents.

• Silica gel only.

• Only inorganic salts.

• Agarose, acrylamide, or other compounds.