Size Exclusion Chromatography: Example #1

The video tutorial explains size exclusion chromatography, focusing on determining molecular weights using a calibration curve. It covers plotting log molecular weight against elution volume, deriving a regression line, and using this to calculate unknown protein weights. The process involves understanding the calibration curve, applying the regression equation, and converting logarithmic values to actual molecular weights.

What is the purpose of plotting the log of molecular weight against elution volume in size exclusion chromatography?

• To find the molecular weight of an unknown protein

• To determine the concentration of the protein

• To calculate the volume of the column

• To measure the purity of the sample

In the regression equation Y = -0.4X + 4.52, what does the variable X represent?

• Concentration of the protein

• Elution volume

• Column length

• Log of molecular weight

How is the molecular weight of an unknown protein determined using the regression equation?

• By determining the purity of the sample

• By calculating the area under the curve

• By using the elution volume in the regression equation

• By measuring the concentration of the protein

What is the next step after obtaining the log of the molecular weight from the regression equation?

• Divide by the slope of the line

• Take the antilogarithm to find the molecular weight

• Multiply by the elution volume

• Subtract the Y-intercept

Why is it important to use molecular weight standards in size exclusion chromatography?

• To enhance the resolution of the chromatogram

• To increase the speed of the chromatography process

• To calibrate the column for accurate measurements

• To reduce the cost of the experiment