Nucleotide Metabolism | Purine Biosynthesis 2: IMP to AMP & GMP

The video explains the biosynthesis of adenylate (AMP) and guanylate (GMP) from inosine monophosphate (IMP). It details the enzymatic pathways for converting IMP into AMP and GMP, highlighting the role of enzymes like adenylosuccinate synthetase and IMP dehydrogenase. The video also discusses the regulatory mechanisms that balance AMP and GMP synthesis, emphasizing allosteric regulation and feedback inhibition. The importance of maintaining a balance between purine nucleotides is underscored, with ATP and GTP playing crucial roles in this regulation.

Which enzyme is responsible for converting IMP to AMP?

• Adenylosuccinate synthetase

• IMP dehydrogenase

• GMP synthetase

• XMP reductase

What is the intermediate nucleotide formed during the conversion of IMP to GMP?

• XMP

• AMP

• ATP

• GTP

How does AMP regulate its own synthesis?

• By activating adenylosuccinate synthetase

• By inhibiting adenylosuccinate synthetase

• By activating IMP dehydrogenase

• By inhibiting GMP synthetase

What happens when GMP levels are high in the cell?

• GMP synthesis is increased

• AMP synthesis is decreased

• IMP is diverted to AMP synthesis

• IMP is converted to XMP

Which molecule is used as a phosphate source in the synthesis of AMP?

• NADH

• XMP

• GTP

• ATP

What is the role of ATP in GMP synthesis?

• It acts as a substrate in GMP synthetase

• It inhibits GMP synthetase

• It is not involved in GMP synthesis

• It is converted to GTP

How does the cell ensure a balance between AMP and GMP levels?

• By using ATP for both AMP and GMP synthesis

• By using GTP for both AMP and GMP synthesis

• By converting AMP directly to GMP

• Through feedback inhibition and substrate utilization