Amylase and pH Experiment: Observing and Collecting Results

The video tutorial demonstrates an experiment to observe the effect of pH on amylase activity. Catherine conducts the experiment using various buffer solutions, starch, and amylase, while maintaining a constant temperature. The process involves setting up a water bath, preparing spotting tiles, and testing for starch presence at different time intervals. Results are recorded for buffer solutions with pH values of 5, 6, 7, and 8, showing how pH affects the rate of starch digestion.

What is the primary function of a buffer solution in the experiment?

• To increase the temperature of the solution

• To act as a catalyst in the reaction

• To maintain a stable pH regardless of environmental changes

• To change the pH of the solution

Why is it important to use separate pipettes for each solution?

• To ensure accurate measurement of solutions

• To avoid cross-contamination between solutions

• To prevent the solutions from evaporating

• To save time during the experiment

What is the purpose of the Bunsen burner in setting up the water bath?

• To mix the solutions thoroughly

• To heat the water to a specific temperature

• To sterilize the equipment

• To provide light for the experiment

What is the role of iodine solution in the experiment?

• To maintain the temperature

• To increase the reaction rate

• To test for the presence of starch

• To act as a buffer

At what point does Catherine start the stopwatch during the experiment?

• After adding the buffer solution

• After adding the starch solution

• After adding the amylase solution

• After placing the test tube in the water bath

What indicates that all the starch has been digested in the test tube?

• The iodine solution remains its normal color

• The iodine solution turns blue-black

• The test tube becomes cloudy

• The temperature of the water bath increases

How long did it take for the starch to be digested at pH 5?

• 3 minutes

• 2.5 minutes

• 2 minutes

• 1 minute

What was the recorded time for starch digestion at pH 6?

• 2 minutes

• 30 seconds

• 1 minute

• 1.5 minutes

At which pH level did the starch take the longest time to digest?

• pH 5

• pH 6

• pH 7

• pH 8

What is the significance of repeating the experiment with different pH levels?

• To test the accuracy of the iodine solution

• To ensure the equipment is functioning properly

• To determine the effect of temperature on amylase activity

• To observe the effect of pH on starch digestion