Using the Light Microscope: Observing Onion Cells and Estimating Magnification

What is the primary function of a light microscope?

• To produce sound waves

• To magnify small objects

• To generate electricity

• To measure temperature

Which part of the microscope is used to view the sample?

• Mirror

• Eyepiece

• Stage

• Objective lens

What is the purpose of using iodine in preparing an onion cell slide?

• To magnify the cells

• To clean the slide

• To dry the sample

• To stain the cells for better visibility

Why are inner layers of the onion used for slide preparation?

• They are thicker

• They are more colorful

• They provide better samples for viewing

• They are easier to cut

What is the first step in focusing a microscope?

• Using the fine adjustment

• Changing the light source

• Rotating the stage

• Using the coarse adjustment

At what magnification can you start to see the nucleus inside the onion cell?

• 10x

• 400x

• 100x

• 40x

How is the actual size of an onion cell calculated using magnification?

• By multiplying the image size by magnification

• By dividing the image size by magnification

• By subtracting magnification from the image size

• By adding the image size to magnification

What is a key advantage of electron microscopes over light microscopes?

• They are more portable

• They provide greater magnification and resolution

• They use less electricity

• They are cheaper

What does resolution refer to in microscopy?

• The ability to distinguish between two close objects

• The speed of image capture

• The color accuracy of the image

• The ability to magnify objects

What is the typical length of a dust mite as seen under an electron microscope?

• 16 nanometers

• 16 millimeters

• 16 micrometers

• 16 centimeters