Endocannabinoids [Part 2] | Major Mechanism of Action

The video by Kevin Tolkoff explores the mechanism of action of endocannabinoids, focusing on their synthesis from phospholipids and their role in regulating neurotransmitter release. It explains the committed step in endocannabinoid synthesis, the role of calcium in activating NaCl transferase, and the negative feedback mechanism to prevent excitotoxicity. The video also covers synaptic transmission, enzymatic pathways, retrograde signaling, and the regulation of neurotransmitter release through G protein-coupled receptors.

What is the committed step in endocannabinoid synthesis?

• Phospholipase D activation

• Neurotransmitter binding

• Calcium channel opening

• NaCl transferase activation

How does calcium influx affect neurotransmitter release?

• It increases neurotransmitter release

• It has no effect on neurotransmitter release

• It decreases neurotransmitter release

• It blocks neurotransmitter release

Which enzyme is responsible for the formation of anandamide?

• Calcium channel

• Diacylglycerol lipase

• Phospholipase D

• NaCl transferase

What is the role of endocannabinoids in neurotransmitter release?

• They increase neurotransmitter release

• They have no role in neurotransmitter release

• They regulate neurotransmitter release through negative feedback

• They serve as a positive feedback mechanism

What type of signaling do endocannabinoids primarily use?

• Lateral signaling

• Retrograde signaling

• Direct signaling

• Anterograde signaling

Which G protein is associated with the inhibition of calcium channels?

• Voltage-gated G protein

• G protein-coupled receptor

• Inhibitory G protein (GI)

• Stimulatory G protein (GS)

What is the effect of activating the voltage-gated potassium channel?

• Increased calcium influx

• No change in membrane potential

• Hyperpolarization of the membrane

• Depolarization of the membrane