Lecithinase Test | Theory & Results

The video tutorial explains the less athenase test, which detects bacteria expressing the enzyme less athenase. It covers the theory of lecithins and phospholipids, detailing how less athenase enzymes break down lecithins into diacylglycerol and head groups. The video also describes how to interpret test results, identifying positive results by the presence of a white, opaque precipitate. The practical application of the test using egg yolk agar is discussed, highlighting its role in identifying bacteria.

What are lecithins primarily composed of?

• Nucleic acids

• Proteins

• Carbohydrates

• Phospholipids

What bond does the less athenase enzyme break in phospholipids?

• Between two glycerol molecules

• Between two phosphates

• Between glycerol and phosphorus

• Between two fatty acids

What is a key characteristic of diacylglycerol?

• It is hydrophobic

• It is water-soluble

• It is a protein

• It is hydrophilic

What indicates a positive less athenase test?

• A blue halo

• A white, opaque precipitate

• A clear streak

• No change in the agar

What is the substrate for less athenase in the test?

• Water

• Sugar

• Egg yolk

• Egg white

What happens to phospholipids in a negative less athenase organism?

• They are broken down

• They remain unchanged

• They form a precipitate

• They turn blue

What forms the white opaque border in a positive test?

• Clumps of nucleic acids

• Clumps of carbohydrates

• Clumps of proteins

• Clumps of diacylglycerol