Size Exclusion Chromatography: Example #1

The video tutorial explains size exclusion chromatography, focusing on determining molecular weights using a calibration curve. It covers plotting log molecular weight against elution volume, deriving a regression line, and using this to calculate unknown protein weights. The process involves understanding the calibration curve, applying the regression equation, and converting logarithmic values to actual molecular weights.

Explain the significance of the Y-intercept in the regression equation for size exclusion chromatography.

What steps are involved in generating a standard curve for molecular weight determination?