Performing Column Chromatography

The video tutorial explains chromatography, focusing on column chromatography. It covers the basics of chromatography, the components and preparation of a column, and the process of loading and running samples. The tutorial also discusses the collection and analysis of fractions, and touches on advanced techniques like FPLC. Key concepts include the role of stationary and mobile phases, the importance of solvent management, and the separation of compounds based on polarity.

Explain the concept of wet loading versus dry loading in column chromatography.

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What are the visual indicators that help in monitoring the separation of compounds?

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What is the purpose of collecting fractions during chromatography?

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What techniques can be used to analyze the fractions collected from chromatography?

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