Microbiology: Gram Staining

The video tutorial explains the theory and process of Gram staining, a method used to differentiate between Gram-positive and Gram-negative bacteria based on their cell wall structure. Gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet dye, while Gram-negative bacteria have a thin layer that does not. The tutorial covers the steps of Gram staining, including heat fixation, application of crystal violet, iodine treatment, decolorization, and counterstaining with safranin. The results are interpreted based on the color retained by the bacteria, with Gram-positive appearing purple and Gram-negative appearing pink.

What role does the decolorizer play in the Gram staining process?

What is the purpose of using safranin in the Gram staining procedure?

Summarize the flowchart of the Gram staining process.