Graphical Estimation of Kd from P:L Binding Plot

The video tutorial explains the concept of KD, an equilibrium constant related to protein-ligand binding. It introduces the variable Theta, representing the percentage of binding sites occupied by a ligand. The tutorial demonstrates how to estimate KD graphically from binding curves and compares the binding strength of different ligands. It also presents an alternative method to assess binding strength at a specific concentration, emphasizing that a lower KD indicates stronger binding.

What is the relationship between ligand concentration and the percentage of binding sites occupied?

How does the binding curve differ for two ligands with different KD values?

Describe the process of determining which ligand binds more strongly to a protein.