How does CRISPR work? Feng Zhang explains with a nursery rhyme

The video explains how genetic mutations can cause diseases and introduces CRISPR technology as a tool to edit the human genome. Using an analogy of proofreading a novel, the video describes how CRISPR can precisely edit DNA sequences by replacing incorrect sequences with correct ones. The process involves using CAS9 to locate and cut the DNA at the mutation site, followed by repairing the DNA with a correct sequence. The video concludes with acknowledgments.

What role does CAS9 play in the CRISPR process?

How does the cell repair the DNA break after CAS9 makes a cut?