Hippurate Hydrolysis Test | Theory & Results

The video explains the hippurate hydrolysis test, a method to determine if bacteria can hydrolyze hippurate using the enzyme hippuricase. The process involves breaking down hippurate into benzoic acid and glycine, with the latter reacting with ninhydrin to produce a bluish-violet color, indicating a positive test. The video details the reaction mechanism, the role of enzymes, and how to interpret test results.

How does ninhydrin react with glycine during the test?

What color change indicates a positive result in the hippurate hydrolysis test?

What does a negative test result indicate about the bacterial species?