Microbiology: Acid Fast Test

The video tutorial explains the acid fast test, a method used to identify organisms with waxy, nearly impermeable cell walls, such as Mycobacterium and Nocardia. These organisms are resistant to disinfectants and require special staining techniques. The acid fast test involves staining with carbol fusion, decolorizing, and counterstaining with methylene blue. Acid fast organisms retain the red carbol fusion stain, while non-acid fast organisms appear blue. This test is crucial for diagnosing diseases like tuberculosis.

What is the role of carbol fusion in the acid fast staining process?

How can the acid fast test be used in clinical diagnostics?