The Michaelis Constant, Km: What is it?

The video tutorial explains Michaelis-Menten kinetics, focusing on the concept of KM, which is the substrate concentration needed to achieve half of the VMAX, the maximum rate of an enzyme. It covers the mathematical representation of KM, its units, and provides a proof of its definition. The tutorial also discusses practical methods to determine KM, such as Lineweaver-Burk and Haynes-Wolf plots, and emphasizes the importance of understanding KM in enzyme kinetics.

How can KM be determined experimentally?

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Discuss the derivation of the Michaelis-Menten equation and its components.

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What does it mean when the substrate concentration is equal to KM?

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