Gene Expression Analysis and DNA Microarray Assays

The video tutorial explains the importance of understanding gene expression in biology, focusing on techniques like RT-PCR and DNA microarray assays. It covers how these methods help analyze gene expression in different contexts, such as embryonic development, tissue analysis, and cancer research. The tutorial highlights the process of reverse transcription to create cDNA from mRNA and the use of DNA microarrays to study genome-wide expression, providing insights into gene activity and potential mutations.

Discuss the importance of fluorescent labeling in DNA microarray assays.

What conclusions can be drawn from the presence or absence of fluorescence in a DNA microarray?

What information can be obtained by comparing mRNAs from normal and cancer cells using microarrays?