Protein:Ligand Saturation Equation: Example #2

The video tutorial explains how to determine ligand concentration using a formula involving Theta, the percentage of protein binding sites occupied by the ligand, and the dissociation constant (KD). The instructor demonstrates the process of rewriting the formula to solve for ligand concentration, providing a step-by-step calculation example. The tutorial concludes with a brief introduction to the Hill plot, which will be covered in the next video.

Explain the significance of the dissociation constant (KD) in determining ligand concentration.

What is the final ligand concentration calculated in the example, and what units is it expressed in?