Protein Purification Table Calculations: Formulas, No Numbers

This video tutorial covers the steps to create a purification table, focusing on enzyme purification and activity assays. It explains the Bradford assay for determining protein concentration and details the calculations for enzyme concentration, activity per milliliter, specific activity, and total enzyme units. The video aims to clarify common misunderstandings in biochemistry labs and sets the stage for further discussions on percent yield and fold purity in subsequent videos.

What is the purpose of creating a purification table in biochemistry?

Explain the significance of enzyme assays in the purification process.

Describe the Bradford assay and its role in protein quantification.

Explain the relationship between absorbance and concentration in the context of the Bradford assay.

How do you calculate the concentration of an enzyme after performing a Bradford assay?

What is the importance of recording dilution factors during enzyme assays?

What is the significance of the extinction coefficient in enzyme assays?

How do you determine the specific activity of an enzyme?

What calculations are necessary to find the total enzyme activity from the purification process?

Discuss the steps involved in calculating percent yield and fold purity in enzyme purification.