Column Chromatography

The video tutorial explains column chromatography, a technique used to separate and purify compounds in a mixture. It covers the setup of the column, loading and running the sample, and collecting fractions. The process involves using silica gel as a stationary phase and a solvent system to separate components based on their interactions. The tutorial also discusses analyzing fractions using TLC to identify the desired product. It concludes with encouragement for beginners to practice and learn from experienced lab mates.

What steps should be taken to ensure that the sample is loaded correctly onto the column?

Discuss the importance of collecting fractions during the chromatography process.

How can the polarity of the eluent be adjusted during the chromatography process?

What should be done with the fractions after they are collected?