tRNA Processing 2: Splicing

The video tutorial explains the complex process of tRNA splicing, which involves five key enzymes: tRNA endonuclease, cyclic phosphodiesterase, adenylate synthetase, exon ligase, and 2' phosphotransferase. The process begins with the initial substrate, an intermediate tRNA, and involves the removal of introns and the joining of exons to form a mature tRNA. Each enzyme plays a specific role in modifying the tRNA structure, ultimately leading to the formation of an anticodon loop that interacts with mRNA codons during protein synthesis.

What happens during the action of adenylate synthetase in the splicing mechanism?

How does the 2 prime phosphotransferase enzyme contribute to the tRNA maturation process?

What is the significance of the anticodon loop formed after the splicing of tRNA?