Detoxification of Cyanide by Thiosulfate Sulfotransferase: Physiology and Mechanism

The video tutorial explores the enzyme rhodanese, also known as thiosulfate sulfotransferase, which performs two main functions: biosynthesis of thiosulfate and detoxification of hydrogen cyanide. The enzyme's mechanism involves thioredoxin dependency and active site interactions with arginine residues. The process results in the formation of thiocyanate, which is excreted from the body. The tutorial provides a detailed explanation of the enzyme's role in detoxifying cyanide and the biochemical pathways involved.

How does the structure of thiosulfate differ from that of sulfate?

What are the implications of having cyanide in the body, and how does rhodanese help mitigate these effects?

Discuss the significance of the arginine residues in the active site of rhodanese.

What is the final product of the reaction between rhodanese and cyanide?