Mastering Optical Microscopes for Cell Observation

This video tutorial provides a comprehensive guide on using an optical microscope to observe cells on a prepared slide. It covers the parts of the microscope, including the stage, objective lenses, and eyepiece, and explains how to use the coarse and fine focusing dials. The tutorial also demonstrates how to calculate total magnification and offers tips for observing animal and plant cells. Additionally, it emphasizes the importance of this practical skill for exams and provides guidance on making labeled drawings of cells.

What is the main objective of this video tutorial?

• To understand how to use an optical microscope to view cells

• To learn about different types of microscopes

• To explore the history of microscopes

• To compare optical and electron microscopes

Which part of the microscope holds the slide in place?

• Objective lens

• Fine focusing dial

• Eyepiece

• Stage clips

What is the function of the mirror in some optical microscopes?

• To magnify the image

• To reflect light up through the slide

• To hold the slide in place

• To adjust the focus

What should you do first when using an optical microscope to view a slide?

• Adjust the fine focusing dial

• Place the slide on the stage and secure it with clips

• Select the highest power objective lens

• Look through the eyepiece

Why is it important to look at the microscope from the side while adjusting the objective lens?

• To ensure the light is properly aligned

• To adjust the magnification

• To avoid damaging the slide

• To see the cells more clearly

How do you calculate the total magnification of an optical microscope?

• Subtract the magnification of the eyepiece from the objective lens

• Divide the magnification of the objective lens by the eyepiece

• Multiply the magnifications of the eyepiece and objective lens

• Add the magnifications of the eyepiece and objective lens

What is the total magnification if the eyepiece lens is 10x and the low-power objective lens is 4x?

• 400x

• 4x

• 40x

• 14x

Which of the following structures can be seen in animal cells using an optical microscope?

• Endoplasmic reticulum

• Ribosomes

• Golgi apparatus

• Nucleus

What additional structures might be visible in plant cells under an optical microscope?

• Endoplasmic reticulum and Golgi apparatus

• Ribosomes and mitochondria

• Chloroplasts and vacuole

• Lysosomes and centrioles

What should be included in your drawing of cells observed under a microscope?

• The name of the observer

• A scale bar and magnification

• The date of observation

• The type of microscope used