Quantifying Bacterial Concentration Using Serial Dilution and Plate Count Method

Quantifying Bacterial Concentration Using Serial Dilution and Plate Count Method

Assessment

Interactive Video

Biology, Chemistry, Science

9th - 10th Grade

Practice Problem

Hard

Created by

Patricia Brown

FREE Resource

This video tutorial teaches how to quantify bacterial concentration using serial dilution and plate count methods. It covers labeling microcentrifuge tubes and LB agar plates with dilution factors, performing serial dilutions using micropipettes, and plating the diluted cultures. The tutorial emphasizes aseptic techniques and concludes with instructions for incubating the plates for analysis.

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9 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary method discussed in the video for quantifying bacterial concentration?

Flow cytometry

Spectrophotometry

Serial dilution and plate count

Gram staining

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How many sterile microcentrifuge tubes should be labeled for the experiment?

6

7

5

8

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What volume of LB broth is pipetted into each microcentrifuge tube?

900 microliters

500 microliters

1000 microliters

100 microliters

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of vortexing or pipetting up and down during the dilution process?

To sterilize the culture

To decrease bacterial concentration

To ensure even mixing

To increase bacterial growth

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the correct technique for spreading the bacterial culture on the agar plate?

Spread plate technique

Pour plate technique

Drop plate technique

Streak plate technique

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why should the inoculation loop or bacterial spreader be flamed before use?

To heat the culture

To sterilize it

To cool it down

To mark the plate

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what temperature should the plates be incubated?

25 degrees Celsius

30 degrees Celsius

37 degrees Celsius

42 degrees Celsius

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