How to Use a Microscope

Professor Murry Gans introduces the non-majors biology lab at Eastfield College, focusing on the use of a light microscope. The video covers handling, parts, and functions of the microscope, preparing a wet mount slide, focusing techniques, and proper storage. It emphasizes the importance of starting with low power and gradually increasing magnification while centering the specimen. The video concludes with a review of key points and encourages students to come prepared to class.

What is the main purpose of the video tutorial presented by Professor Murry Gans?

• To teach students how to use a light microscope

• To explain the history of microscopes

• To demonstrate advanced biological experiments

• To introduce students to the biology lab

Which part of the microscope magnifies the specimen 10 times?

• The diaphragm

• The 4x objective lens

• The eyepieces or oculars

• The 40x objective lens

What is the function of the diaphragm on a microscope?

• To adjust the focus of the image

• To control the amount of light reaching the specimen

• To magnify the specimen

• To hold the slide in place

What is the first step in preparing a wet mount slide?

• Adding a cover slip

• Cleaning the lenses

• Adjusting the diaphragm

• Placing the specimen on the slide

Why is it important to avoid air bubbles when preparing a wet mount slide?

• They can damage the microscope

• They make the slide heavier

• They can obscure the view of the specimen

• They increase the magnification

Why do we start with the low power objective when focusing a microscope?

• It provides the highest magnification

• It is the default setting

• It has the largest field of view

• It is easier to clean

What should you do if you cannot see the specimen clearly at high power?

• Use a different slide

• Clean the lenses again

• Switch back to low power and recenter the specimen

• Increase the light intensity

What is the correct way to carry a microscope?

• By the objective lenses

• By the arm and supporting the base

• By the base only

• By the eyepieces

What should be done with the cover slip after using the microscope?

• Leave it on the microscope

• Store it in the slide box

• Dispose of it as instructed

• Wash and reuse it

What is a key takeaway from the video regarding preparation for lab classes?

• Watch the video multiple times

• Read the chapter and lab procedure before class

• Always bring your own microscope

• Focus on memorizing the parts of the microscope