Enzyme Reaction Rate Measurement Using Catalase and Hydrogen Peroxide

This video tutorial demonstrates how to determine the rate of a reaction using yeast catalase and hydrogen peroxide. The instructor explains the setup, including spherified catalase spheres and a liquid substrate. The rate is measured in millimeters per second by observing the height of the liquid. The experiment involves dropping a sphere into the liquid, starting a timer when it sinks, and stopping it when the sphere floats back up. The process is repeated for five trials, with instructions to record the results. The video also includes a humorous note about the instructor's dog snoring in the background.

What enzyme is used in the experiment to determine the reaction rate?

• Amylase

• Catalase

• Protease

• Lipase

What is the substrate used in the experiment?

• Carbon dioxide

• Water

• Oxygen

• Hydrogen peroxide

In what units should the height of the liquid be recorded?

• Centimeters

• Inches

• Meters

• Millimeters

What is the purpose of using a ruler in the experiment?

• To measure the diameter of the sphere

• To measure the height of the liquid

• To measure the temperature of the liquid

• To measure the weight of the sphere

What action should be taken when the sphere reaches the bottom of the liquid?

• Stop the timer

• Start the timer

• Remove the sphere

• Add more substrate

What indicates that the reaction has produced enough oxygen?

• The sphere floats to the top

• The sphere dissolves

• The liquid evaporates

• The liquid changes color

How many times is the experiment repeated in the video?

• Five times

• Three times

• Seven times

• Nine times

What should you do if you need more time to record your numbers during the trials?

• Rewind the video

• Fast forward the video

• Pause the video

• Skip the video