Cell Culture Techniques and Safety

The video tutorial explains the aseptic technique to prevent contamination in cell cultures. It covers preparation steps, including hand washing and wearing PPE, cleaning the cell culture hood with disinfectant and ethanol, and preparing media aseptically. Proper handling and labeling of media bottles are also discussed to minimize contamination risks.

What is the primary purpose of aseptic technique in cell culture?

• To increase nutrient absorption

• To improve cell visibility

• To enhance cell growth

• To prevent contamination

Which of the following is NOT part of the personal protective equipment required before working in a cell culture hood?

• Safety glasses

• Lab coat

• Face mask

• Gloves

What should be used to clean the inside of the cell culture hood before use?

• Soap and water

• 70% ethanol

• Bleach

• Vinegar

Why is it important to warm cell culture media before use?

• To match the temperature of the incubator

• To improve solubility of nutrients

• To activate the supplements

• To sterilize the media

What is the correct way to handle the lid of a bottle during aseptic preparation?

• Hold it in your hand

• Place it on the table

• Ensure the opening is facing downwards

• Leave it open

What should you do immediately after adding supplements to the media?

• Shake the bottle

• Store it in the fridge

• Replace the lid

• Label the bottle

How should you label the media bottle after preparation?

• With your initials, date, and contents

• With the time of preparation only

• With the lab supervisor's name

• With the experiment name only

What is a key action to avoid when working with open bottles to prevent contamination?

• Placing the lid on the table

• Sweeping hands over the bottle

• Using a new pipette tip

• Warming the media