Gene Expression and Spinal Cord Analysis

The video tutorial provides a technical tour of the Allen Spinal Cord Atlas, demonstrating its basic search and navigation features. It explains how to access the Atlas, details the gene expression patterns in C-57 black mice, and describes the manual curation process. The tutorial covers how to search for genes, view data using zoom and pan viewers, and analyze gene expression with various tools. It also shows how to access experiment metadata and download high-resolution images. The video concludes with acknowledgments and directs viewers to additional resources.

What is the primary focus of the Allen Spinal Cord Atlas?

• Developing new spinal cord therapies

• Analyzing spinal cord injuries

• Studying gene expression in adult and juvenile mice

• Mapping gene expression in human brains

How is the gene expression data in the Spinal Cord Atlas curated?

• Automatically by AI algorithms

• Manually by anatomists

• Through public contributions

• By using MRI scans

What information is provided in the list of experiments returned from a search?

• Only the gene symbol and name

• Gene symbol and experiment cost

• Gene symbol, gene name, mouse age, and image representation

• Mouse age and experiment duration

How many sections are captured in a single gene expression assay?

• 20 sections

• 16 sections

• 12 sections

• 8 sections

What feature allows you to search for genes with specific criteria?

• Advanced search

• Filtered search

• Basic search

• Quick search

What tool helps to visualize low and high gene expression levels?

• Gene expression chart

• Image filter

• Color gradient tool

• Expression mask view

Which data set can show the architecture of the spinal cord?

• Nissle and acetel colon esteras data sets

• Gene expression data

• Experimental metadata

• Annotated Atlas

What does the TR9 region refer to in the annotated Atlas?

• Triceps motor neurons of Lamina 9

• Thoracic region 9

• Temporal region 9

• Tissue region 9

What can you do to better localize gene expression in the spinal cord?

• Rotate the image

• Sync viewers to a similar position

• Use the zoom and pan viewer

• Change the color scheme

What additional information can be accessed from the metadata page?

• Mouse behavioral data

• Spinal cord injury statistics

• Gene therapy options

• Experimental images and conditions