Thin Layer Chromatography Techniques

This video tutorial explains the process of thin layer chromatography (TLC), a technique used to separate components of a mixture. It covers the preparation of a TLC plate, loading samples using a micro capillary tube, and setting up the TLC chamber with a solvent mixture. The video also demonstrates how to develop the TLC plate and observe the results, including using a UV lamp if the sample is not colored.

What is the primary purpose of using Thin Layer Chromatography?

• To mix different components of a solution

• To separate different components of a mixture

• To measure the density of a solution

• To change the color of a solution

Which side of the TLC plate should be used for the experiment?

• The side with no coating

• Either side can be used

• The rough side

• The smooth side

How far from the bottom of the TLC plate should the start line be drawn?

• 1 cm

• 3 cm

• 5 cm

• 2 cm

What tool is used to load the sample onto the TLC plate?

• A pipette

• A micro capillary tube

• A syringe

• A dropper

Why is it important to keep the sample spot as small as possible?

• To maximize resolution

• To save space on the plate

• To use less sample

• To make it easier to see

What is the typical solvent mixture used in the TLC chamber?

• 3:7 mixture of hexane and acetone

• 1:1 mixture of hexane and acetone

• 7:3 mixture of hexane and acetone

• 5:5 mixture of hexane and acetone

What should be done after placing the TLC plate in the developing chamber?

• Add more solvent

• Leave the chamber open

• Cap the chamber immediately

• Shake the chamber

What should you do if the solvent line is not visible?

• Remove the plate

• Use a magnifying glass

• Add more solvent

• Shade the chamber with one hand

What is the next step after the solvent line reaches the top line?

• Add more solvent

• Remove the plate and let it dry

• Reapply the sample

• Start a new experiment

What tool is used to observe non-colored samples on the TLC plate?

• A magnifying glass

• A UV lamp

• A flashlight

• A microscope