CRISPR-Cas9 and Genetic Engineering

The video explores genetic engineering, focusing on DNA's role in genetic traits and the CRISPR-Cas9 system. It explains DNA structure, genetic modification applications, and CRISPR's potential in medicine. Ethical concerns and future improvements in CRISPR technology are also discussed.

What is the primary function of DNA in living organisms?

• To protect against diseases

• To transport nutrients

• To carry genetic instructions

• To provide energy

Which of the following is NOT a nucleobase found in DNA?

• Cytosine

• Guanine

• Adenine

• Uracil

What is the role of restriction enzymes in genetic engineering?

• To repair DNA

• To synthesize proteins

• To cut DNA at specific sites

• To replicate DNA

How is insulin produced using genetic engineering?

• By extracting it from plants

• By synthesizing it chemically

• By harvesting it from animals

• By using genetically modified bacteria or yeast

What is the primary function of the Cas9 protein in the CRISPR system?

• To bind to RNA

• To cut DNA strands

• To replicate DNA

• To repair DNA

What is the role of guide RNA in the CRISPR-Cas9 system?

• To cut DNA

• To repair DNA

• To bind to the target DNA sequence

• To replicate DNA

Why is editing germline cells with CRISPR-Cas9 controversial?

• It is illegal in most countries

• It is ineffective

• It is too expensive

• Changes are passed to future generations

What is a major challenge in using CRISPR-Cas9 for gene editing?

• Off-target effects

• Limited applications

• High cost

• Lack of precision

Which countries have started using CRISPR-Cas9 to cure diseases?

• US and China

• India and Japan

• Brazil and Australia

• Germany and France

What is one proposed method to improve the accuracy of CRISPR-Cas9?

• Designing better guide RNAs

• Using more enzymes

• Increasing the number of cuts

• Reducing the size of Cas9