Microscope Operation and Maintenance

This video tutorial guides viewers through the process of setting up and using a microscope. It covers initial setup steps, including plugging in the microscope and adjusting the lighting. The tutorial explains how to position and center a slide on the stage, introduces different objective lenses, and demonstrates focusing techniques using coarse and fine focus knobs. It also covers the use of immersion oil for high magnification and concludes with instructions on cleaning and storing the microscope.

What is the first step in setting up the microscope?

• Using the fine focus knob

• Placing the slide

• Plugging in the microscope

• Adjusting the stage

How should the rheostat be initially set when turning on the illuminator?

• Fully open

• Fully closed

• Halfway open

• Three-quarters open

What should you do after lowering the stage all the way to the bottom?

• Turn on the illuminator

• Place the slide on the stage

• Use the fine focus knob

• Adjust the iris diaphragm

Which objective is referred to as the scanning objective?

• 40x objective

• 10x objective

• 100x objective

• 5x objective

What should you do after rotating the turret to the lowest power objective?

• Open the iris diaphragm fully

• Close the iris diaphragm fully

• Slightly open the iris diaphragm

• Use the fine focus knob

When should you switch from using the coarse focus knob to the fine focus knob?

• After cleaning the slide

• After focusing with the lowest power objective

• After centering the specimen

• After placing the slide

What is the risk of using the coarse focus knob with higher magnifications?

• It could move the stage too slowly

• It could damage the slide and the scope

• It could blur the image

• It could increase the light intensity

What should be done before using the 100x objective?

• Remove the slide

• Use the coarse focus knob

• Lower the stage

• Add a drop of immersion oil

Why is it important to clean the immersion oil off the 100x objective?

• To reduce light intensity

• To prevent damage to the 40x objective

• To ensure clear viewing for the next use

• To avoid contamination of the slide

What should be the final step after viewing the specimen?

• Clean the microscope

• Move the stage all the way down

• Turn off the illuminator

• Remove the slide