Microscope Slide Preparation Techniques

This video tutorial demonstrates how to prepare and observe a transverse section of a dicot stem. It begins with an introduction to the materials needed, followed by detailed steps on how to cut thin sections of the stem, stain them using saffron, and mount them on slides with glycerin. Finally, the tutorial shows how to observe the prepared sections under a microscope, highlighting the cellular structures visible in the dicot stem.

What is the first step in preparing a TS of a dicot stem?

• Hydrating the preserved stem

• Staining the sections

• Observing under a microscope

• Placing the cover slip

Why is it important to make thin sections of the dicot stem?

• To prevent dehydration

• To choose the finest section for observation

• To allow for better staining

• To ensure they fit on the slide

Which material is used to stain the dicot stem sections?

• Saffron

• Glycerin

• Filter paper

• Water

What is the purpose of using glycerin in the slide preparation?

• To preserve the stained section

• To prevent air bubbles

• To hydrate the sections

• To stain the cells

What is the role of the filter paper in the slide preparation process?

• To remove excess stain

• To hydrate the sections

• To support the cover slip

• To clean the slide

How should the cover slip be placed on the slide?

• With force to remove air bubbles

• Gently at a 45-degree angle

• At a 90-degree angle

• Directly from above

Why is it important to avoid air bubbles when placing the cover slip?

• They are necessary for observation

• They hydrate the section

• They enhance the stain

• They can distort the image

What is the magnification used to observe the dicot stem under the microscope?

• 5x

• 10x

• 20x

• 40x

Which structure is visible in the TS of the dicot stem under the microscope?

• Chloroplasts

• Stomata

• Vascular bundles

• Root hairs

What is the purpose of observing the dicot stem under a microscope?

• To identify the plant species

• To measure the stem diameter

• To see the internal structure

• To count the number of cells