Preparing Wet Mount Slides

This video tutorial teaches how to make a wet mount slide for viewing specimens under a microscope. It covers the materials needed, such as a glass slide, cover slip, and dropper bottle, and provides step-by-step instructions on preparing the slide, placing the specimen, and adjusting the cover slip to avoid air bubbles. The tutorial concludes with the importance of this technique for a photosynthesis lab.

What is the primary purpose of a wet mount slide?

• To dry out specimens for preservation

• To prepare specimens for viewing under a microscope

• To clean the microscope lens

• To store specimens for long-term study

Which of the following is NOT a material needed for preparing a wet mount slide?

• Glass slide

• Cover slip

• Microscope lens

• Dropper bottle

What is the first step in preparing a wet mount slide?

• Filling the dropper bottle

• Placing the cover slip

• Cleaning the glass slide

• Adding the specimen

How many drops of water are typically added to the slide?

• Four to five

• One

• Two to three

• Six to seven

What should you do after placing the specimen on the slide?

• Ensure the specimen is centered

• Dry the slide

• Immediately place the cover slip

• Add more water

What is the correct way to position the cover slip initially?

• Place it on the specimen

• Hold it at an angle

• Stand it straight up

• Lay it flat immediately

Why is it important to use a pencil or pen when lowering the cover slip?

• To measure the thickness of the specimen

• To write notes on the slide

• To prevent the cover slip from falling

• To help lower the cover slip gently

What should you do if the cover slip is held up by the specimen?

• Add more water

• Remove the specimen

• Tilt the slide

• Gently press down with a pencil

Why is it important to avoid air bubbles under the cover slip?

• They cause the slide to dry out

• They can be mistaken for specimen features

• They make the slide heavier

• They can damage the specimen

What is the final step in preparing a wet mount slide?

• Labeling the slide

• Checking for air bubbles

• Adding more water

• Removing excess water