Microscopy Measurement Techniques

This tutorial explains how to calibrate an ocular micrometer in a microscope. It covers the importance of calibration, the process of using a stage micrometer, and how to calculate measurements in microns. The tutorial emphasizes the need for individual calibration for each objective lens and provides practice examples to reinforce learning.

What is the primary function of an ocular micrometer in microscopy?

• To magnify the specimen

• To provide a scale for measuring small specimens

• To adjust the focus of the microscope

• To illuminate the specimen

Why is it impractical to use a regular ruler for measuring microscopic specimens?

• Microscopes do not have enough space for a regular ruler

• Regular rulers are too expensive

• Regular rulers can damage the specimen

• Microscopic specimens are too small to be measured with a regular ruler

How does changing the objective lens affect the measurement in ocular units?

• It does not affect the measurement

• It changes the physical size of the specimen

• It changes the color of the specimen

• It alters the number of ocular units the specimen spans

What is the purpose of using a stage micrometer in the calibration process?

• To clean the microscope

• To provide a known measurement for comparison

• To increase the magnification

• To adjust the lighting

What is the equivalent of one stage unit in microns?

• 10 microns

• 100 microns

• 1000 microns

• 1 micron

How many microns does one ocular unit equal when using the 10x objective lens?

• 101 microns

• 98 microns

• 100 microns

• 99 microns

Why is it important to record the calibration result in your lab notebook?

• To remember the magnification settings

• To ensure the microscope is clean

• To have a reference for future measurements

• To adjust the lighting correctly

What is the equivalent of one ocular unit in microns when using the 60x objective lens?

• 170 microns

• 100 microns

• 99 microns

• 17 microns

Why must each objective lens be calibrated individually?

• Because each lens has a different color

• Because each lens has a different magnification

• Because each lens is used for different specimens

• Because each lens is made of different materials

What happens to the field of view when using a higher magnification lens like 60x?

• It decreases

• It increases

• It remains the same

• It becomes blurry