Recombinant DNA Technology Concepts

The video tutorial explains the use of plasmids and Lambda phage as vectors for recombinant DNA. It covers the lytic and lysogenic cycles of Lambda phage, detailing how these cycles can be harnessed for DNA cloning. The tutorial also provides practical steps for replacing Lambda phage DNA with target DNA, using restriction enzymes and DNA ligase, to produce recombinant DNA for genetic engineering purposes.

What is the primary role of bacterial plasmids in recombinant DNA technology?

• To produce viral proteins

• To integrate into the host genome

• To act as vectors for DNA molecules

• To destroy viral DNA

Which type of bacteriophage is known for infecting E. coli cells?

• T4 Phage

• Phi X 174

• M13 Phage

• Lambda Phage

What happens to a bacterial cell during the lytic cycle?

• It undergoes binary fission

• It produces viral particles and bursts

• It integrates viral DNA into its genome

• It becomes dormant

In the lysogenic cycle, what happens to the viral DNA?

• It remains outside the host cell

• It integrates into the host genome

• It is destroyed by the host cell

• It immediately produces viral particles

What triggers the switch from the lysogenic to the lytic cycle?

• Presence of antibiotics

• Stressful environmental factors

• Optimal environmental conditions

• A lack of nutrients

How can Lambda phages be used as vectors for DNA molecules?

• By replacing their DNA with target DNA

• By integrating into the host genome

• By producing viral proteins

• By destroying the host cell

What enzyme is used to cut DNA at specific sites for recombinant DNA technology?

• DNA polymerase

• Restriction enzyme

• RNA ligase

• Helicase

What is the role of DNA ligase in creating recombinant DNA?

• To cut DNA strands

• To synthesize RNA

• To join DNA fragments

• To replicate DNA

What must be true about the size of the target DNA when using Lambda phages as vectors?

• Size does not matter

• It must be larger than the original DNA

• It must be smaller than the original DNA

• It must be the same size as the original DNA

What is the outcome of using Lambda phages as vectors in recombinant DNA technology?

• Production of viral proteins

• Integration into the host genome

• Destruction of the host cell

• Amplification of the target DNA