Microscopy Techniques and Cell Preparation

The video tutorial demonstrates how to prepare a wet mount slide of eukaryotic animal cells, specifically epithelial cells from the inside of the cheek. It covers the materials needed, the process of collecting and staining the sample, and how to apply a cover slip. The video also shows how to observe the cells under a microscope at different magnifications, highlighting the appearance of cheek cells and oral bacteria. The tutorial concludes with a mention of additional resources available online.

What type of cells are being prepared in the wet mount slide in this video?

• Bacterial cells

• Plant cells

• Eukaryotic animal cells

• Prokaryotic cells

Why is a 9% sodium chloride solution used instead of water in the preparation?

• To increase the slide's durability

• To stain the cells

• To prevent cell bursting due to osmotic pressure

• To enhance cell visibility

What tool is used to collect epithelial cells from the cheek?

• Tweezers

• Cotton swab

• Microscope slide

• Toothpick

Why must the used toothpick and paper towel be disposed of properly?

• They are biohazards

• They are recyclable

• They are expensive

• They are reusable

What is the purpose of using methylene blue in the preparation?

• To disinfect the sample

• To prevent air bubbles

• To clean the slide

• To stain the cells for better visibility

How should the cover slip be applied to avoid air bubbles?

• By dropping it from a height

• By sliding it from the side

• By holding it at a right angle and letting it drop

• By pressing it down firmly

At what magnification do cheek cells appear like little fried eggs?

• 40x

• 100x

• 400x

• 1000x

What is the blue dot observed inside the cheek cells under the microscope?

• Cytoplasm

• Cell membrane

• Nucleus

• Mitochondria

What additional structures are visible at 1000x magnification?

• Chloroplasts

• Oral bacteria

• Cell walls

• Vacuoles

What is the main reason for the cells clumping together under the microscope?

• They are sticky cells of the mucous membrane

• They are over-stained

• They are dead

• They are dehydrated