Settle Blade Technique Procedures

The video tutorial explains the settle plate technique, a method used to monitor environmental conditions and check microbiological quality in laboratories or production areas. It outlines the five steps involved: culture media preparation, settle plate technique, incubation, result observation, and calculation. The necessary equipment includes a balance machine, incubator, biological safety cabinet, autoclave, and trypton soya agar. The video demonstrates how to prepare agar plates, label them, place them in different room sections, and incubate them. After incubation, the video shows how to observe and count bacterial colonies to interpret the results.

What is the primary purpose of the settle blade technique?

• To measure air quality

• To evaluate noise levels in a room

• To monitor environmental conditions for microbiological contamination

• To test chemical reactions

Which of the following is NOT required for the settle blade technique?

• Incubator

• Autoclave

• Microscope

• Balance machine

What is the first step in the settle blade technique?

• Incubation

• Calculation

• Result observation

• Culture media preparation

How should the TSA plates be labeled?

• With the date of the test

• With the name of the person conducting the test

• With the location name of the section

• With the type of agar used

Where should the TSA plates be placed in each section?

• In a corner

• On the floor

• Near a window

• On a table or elevated place

For how long should the room be kept closed during the incubation process?

• 8 hours

• 6 hours

• 4 hours

• 2 hours

At what temperature should the plates be incubated?

• 40 degrees Celsius

• 37 degrees Celsius

• 30 degrees Celsius

• 25 degrees Celsius

What should be done after the incubation period?

• Store the plates in a refrigerator

• Re-label the plates

• Count the bacterial colonies

• Dispose of the plates

How many colonies were found in the East North section?

• Zero

• Seven

• Three

• Five

What is the colony forming unit per square feet in the East South corner?

• Zero

• Three

• Two

• Less than one